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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
22/09/1997 |
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Data da última atualização: |
19/07/2018 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
OLIVEIRA, A. C. de. |
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Afiliação: |
ANTONIO CARLOS DE OLIVEIRA, CNPMS. |
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Título: |
Organização e infra-estrutura de métodos quantitativos em unidades de pesquisa. |
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Ano de publicação: |
1982 |
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Fonte/Imprenta: |
In: ENCONTRO DE MÉTODOS QUANTITATIVOS DA EMBRAPA, 1., 1982. Brasília, DF. Memória... Brasília, DF: EMBRAPA-DMQ, 1982. p. 339-343. |
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Série: |
(EMBRAPA. DMQ/E/02). |
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Idioma: |
Português |
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Thesagro: |
Estatística; Pesquisa. |
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Thesaurus Nal: |
research; statistics. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/53931/1/Organizacao-infra-estrutura.pdf
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Marc: |
LEADER 00578nam a2200157 a 4500
001 1476573
005 2018-07-19
008 1982 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, A. C. de
245 $aOrganização e infra-estrutura de métodos quantitativos em unidades de pesquisa.$h[electronic resource]
260 $aIn: ENCONTRO DE MÉTODOS QUANTITATIVOS DA EMBRAPA, 1., 1982. Brasília, DF. Memória... Brasília, DF: EMBRAPA-DMQ, 1982. p. 339-343.$c1982
490 $a(EMBRAPA. DMQ/E/02).
650 $aresearch
650 $astatistics
650 $aEstatística
650 $aPesquisa
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Embrapa Milho e Sorgo (CNPMS) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
18/08/2015 |
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Data da última atualização: |
15/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ALEMAN GAÍNZA, Y.; DOMINGUES, L. F.; PINO PEREZ, O.; RABELO, M. D.; ROQUE LÓPEZ, E.; CHAGAS, A. C. de S. |
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Afiliação: |
YOUSMEL ALEMAN GAÍNZA, CENSA, MAYABEQUE, CUBA.; LUCIANA FERREIRA DOMINGUES, BOLSISTA, CPPSE, SÃO CARLOS, SP.; ORIELA PINO PEREZ, CENSA, MAYABEQUE, CUBA.; MARCIO DIAS RABELO, CPPSE; EUGENIO ROQUE LÓPEZ, UNIVERSIDAD AGRARIA DE LA HABANA, CUBA.; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Anthelmintic activity in vitro of Citrus sinensis and Melaleuca quinquenervia essential oil from Cuba on Haemonchus contortus. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Industrial Crops and Products, v. 76, p. 647-652, dec. 2015. |
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ISSN: |
0926-6690 |
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DOI: |
https://doi.org/10.1016/j.indcrop.2015.07.056 |
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Idioma: |
Inglês |
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Conteúdo: |
Gastrointestinal nematodes are an important problem in the small ruminant production and their control nearly exclusively depends on commercial anthelmintics. However, parasite resistance to those chemical formulations is an increasing global problem, so new plant-derived compounds are being studied for their potential use against gastrointestinal nematodes. Citrus sinensis and Melaleuca quinquenervia essential oils were evaluated against Haemonchus contortus Embrapa2010 resistant isolate, through the egg hatch test (EHT) and larval development test (LDT) at concentrations ranging from 0.02 mg/mL to 50 mg/mL and from 0.04 mg/mL to 3.12 mg/mL, respectively. All concentrations, positive controls (thiabendazole in EHT or ivermectin in LDT), and negative controls (2% Tween 80 in EHT or 0.5% DMSO in LDT) were performed in six replicates and in three independent experiments, for a total of 18 repetitions involving approximately 1800 parasites per treatment. Significant differences (P ? 0.01) of inhibition percentages were identified by one-way analysis of variance followed by the Tukey test. Inhibitory concentration values (IC50 and IC90) were analyzed by the Probit procedure of SAS. The essential oils evaluated showed that C. sinensis presented limonene as major component (96.0%), while M. quinquenervia contained longifolene (32.95%) and 1,8-cineole (25.43%) as major components. In the EHT, the IC50 and IC90 of the essential oils were respectively 0.27 and 0.99 mg/mL for C. sinensis and, 1.52 and 5.63 mg/mL for M. quinquenervia. In the LDT, the IC50 and IC90 were 0.97 and 2.32 mg/mL for C. sinensis and, 0.44 and 0.94 mg/mL for M. quinquenervia. C. sinensis was five times more effective on eggs than M. quinquenervia. However, it was twice more effective on larvae than C. sinensis, indicating that bioactive essential oils have different modes of action. The results suggest that these compounds are good candidates for nematode control. However, alternative anthelmintics have to be safe to the host, promote parasite control in vivo and not generate residues. So, all these key points need to be elucidated before using any plant extract and/or components. MenosGastrointestinal nematodes are an important problem in the small ruminant production and their control nearly exclusively depends on commercial anthelmintics. However, parasite resistance to those chemical formulations is an increasing global problem, so new plant-derived compounds are being studied for their potential use against gastrointestinal nematodes. Citrus sinensis and Melaleuca quinquenervia essential oils were evaluated against Haemonchus contortus Embrapa2010 resistant isolate, through the egg hatch test (EHT) and larval development test (LDT) at concentrations ranging from 0.02 mg/mL to 50 mg/mL and from 0.04 mg/mL to 3.12 mg/mL, respectively. All concentrations, positive controls (thiabendazole in EHT or ivermectin in LDT), and negative controls (2% Tween 80 in EHT or 0.5% DMSO in LDT) were performed in six replicates and in three independent experiments, for a total of 18 repetitions involving approximately 1800 parasites per treatment. Significant differences (P ? 0.01) of inhibition percentages were identified by one-way analysis of variance followed by the Tukey test. Inhibitory concentration values (IC50 and IC90) were analyzed by the Probit procedure of SAS. The essential oils evaluated showed that C. sinensis presented limonene as major component (96.0%), while M. quinquenervia contained longifolene (32.95%) and 1,8-cineole (25.43%) as major components. In the EHT, the IC50 and IC90 of the essential oils were respectively 0.27 and 0.99 mg/mL for C. sinen... Mostrar Tudo |
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Palavras-Chave: |
HERBAL MEDICINE. |
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Thesagro: |
Citrus Sinensis; Haemonchus Contortus. |
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Thesaurus NAL: |
Cuba; essential oils; Melaleuca quinquenervia. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03069naa a2200277 a 4500
001 2022186
005 2023-03-15
008 2015 bl uuuu u00u1 u #d
022 $a0926-6690
024 7 $ahttps://doi.org/10.1016/j.indcrop.2015.07.056$2DOI
100 1 $aALEMAN GAÍNZA, Y.
245 $aAnthelmintic activity in vitro of Citrus sinensis and Melaleuca quinquenervia essential oil from Cuba on Haemonchus contortus.$h[electronic resource]
260 $c2015
520 $aGastrointestinal nematodes are an important problem in the small ruminant production and their control nearly exclusively depends on commercial anthelmintics. However, parasite resistance to those chemical formulations is an increasing global problem, so new plant-derived compounds are being studied for their potential use against gastrointestinal nematodes. Citrus sinensis and Melaleuca quinquenervia essential oils were evaluated against Haemonchus contortus Embrapa2010 resistant isolate, through the egg hatch test (EHT) and larval development test (LDT) at concentrations ranging from 0.02 mg/mL to 50 mg/mL and from 0.04 mg/mL to 3.12 mg/mL, respectively. All concentrations, positive controls (thiabendazole in EHT or ivermectin in LDT), and negative controls (2% Tween 80 in EHT or 0.5% DMSO in LDT) were performed in six replicates and in three independent experiments, for a total of 18 repetitions involving approximately 1800 parasites per treatment. Significant differences (P ? 0.01) of inhibition percentages were identified by one-way analysis of variance followed by the Tukey test. Inhibitory concentration values (IC50 and IC90) were analyzed by the Probit procedure of SAS. The essential oils evaluated showed that C. sinensis presented limonene as major component (96.0%), while M. quinquenervia contained longifolene (32.95%) and 1,8-cineole (25.43%) as major components. In the EHT, the IC50 and IC90 of the essential oils were respectively 0.27 and 0.99 mg/mL for C. sinensis and, 1.52 and 5.63 mg/mL for M. quinquenervia. In the LDT, the IC50 and IC90 were 0.97 and 2.32 mg/mL for C. sinensis and, 0.44 and 0.94 mg/mL for M. quinquenervia. C. sinensis was five times more effective on eggs than M. quinquenervia. However, it was twice more effective on larvae than C. sinensis, indicating that bioactive essential oils have different modes of action. The results suggest that these compounds are good candidates for nematode control. However, alternative anthelmintics have to be safe to the host, promote parasite control in vivo and not generate residues. So, all these key points need to be elucidated before using any plant extract and/or components.
650 $aCuba
650 $aessential oils
650 $aMelaleuca quinquenervia
650 $aCitrus Sinensis
650 $aHaemonchus Contortus
653 $aHERBAL MEDICINE
700 1 $aDOMINGUES, L. F.
700 1 $aPINO PEREZ, O.
700 1 $aRABELO, M. D.
700 1 $aROQUE LÓPEZ, E.
700 1 $aCHAGAS, A. C. de S.
773 $tIndustrial Crops and Products$gv. 76, p. 647-652, dec. 2015.
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